Journal: Acta Pharmacologica Sinica

Article Title: Brain protection against ischemic stroke using choline as a new molecular bypass treatment

doi: 10.1038/aps.2015.104

Figure Lengend Snippet: Effects of oral choline treatment on angiogenesis in the ischemic brains. (A) Macroscopic images of the pial vasculature. (B) Microscopic images of ischemic cerebral cortex sections immunohistochemically stained using an anti-CD34 antibody to analyze vessel density, scale bar=20 μm. (C) Vessel density (n=6). (D) Number of microvessels in the cortex (n=3). Mean±SEM. bP<0.05, cP<0.01 vs sham; eP<0.05, fP<0.01 vs pMCAO.

Article Snippet: The capillary density was examined using a primary rabbit anti-rat CD34 antibody (Bioss, Beijing, China).

Techniques: Staining

Journal: Brain and Behavior

Article Title: Electrical stimulation improved cognitive deficits associated with traumatic brain injury in rats

doi: 10.1002/brb3.667

Figure Lengend Snippet: Flow cytometry detection of endothelial progenitor cells ( EPC s) in peripheral blood of rats before the traumatic brain injury ( TBI ) (0 day), and 1, 3, 7, 14, 21, 28 days after TBI . They were marked by CD 34 and CD 133. The stress of TBI mobilized the EPC s at 1 day after TBI in N‐ ES group and ES group. Electrical stimulation treatment increased EPC s numbers in peripheral blood from 3 to 21 days after TBI . n = 6/group; * p < .05 ES group versus N‐ ES group; # p < .05 N‐ ES group and ES group versus Sham group

Article Snippet: CD34 + cells in brain tissue were detected using a CD34 antibody (R and D Systems Cat# AF4117 Lot# RRID:AB_2074613 ) as recommended by the manufacturer.

Techniques: Flow Cytometry

Journal: The Journal of Biological Chemistry

Article Title: Sirtuin-1 (SIRT1) Is Required for Promoting Chondrogenic Differentiation of Mesenchymal Stem Cells

doi: 10.1074/jbc.M114.568790

Figure Lengend Snippet: Characterization of MSCs. A, in monolayer culture the human MSCs (panel a) showed a polymorphic, fibroblast-like morphology. The MSCs expressed positive stem cell-specific markers CD90+ (panel b) and CD105+ (panel c) and were negative for the hematopoietic stem cell markers CD45− (panel d) and CD34− (panel e). Magnification: ×10 (panel a), ×40 (panels b–e), and bar, 1 μm. B, to quantify the stem cell marker-positive cells, the labeled cultures were examined by counting 300 cells from 10 microscopic fields. The examination was performed in triplicate, and the results are provided as the mean values with S.D. from three independent experiments. Values were compared with the control, and statistically significant values with p < 0.05 are designated by a star, and p < 0.01 is designated by two stars. Co., without primary antibody, followed by incubation with rhodamine-coupled secondary antibodies and counterstaining with DAPI to visualize the cell nuclei.

Article Snippet: Monoclonal anti-Sox9, monoclonal anti-CD105, monoclonal anti-CD90, monoclonal anti-CD45, and monoclonal anti-CD34 were purchased from Acris Antibodies GmbH (Hiddenhausen, Germany).

Techniques: Marker, Labeling, Incubation

Journal: Advances in Orthopedics

Article Title: Investigating the Possibility of Intervertebral Disc Regeneration Induced by Granulocyte Colony Stimulating Factor-Stimulated Stem Cells in Rats

doi: 10.4061/2011/602089

Figure Lengend Snippet: Some positive BrdU staining around the end plates in the GCSF treatment group, however, no apparent CD34 costaining were disclosed.

Article Snippet: The rat monoclonal anti-CD34 antibody (CD34, 1 : 200, Acris Antibodies GmbH, Hiddenhausen, Germany) was used.

Techniques: BrdU Staining

Journal: Journal of Tissue Engineering and Regenerative Medicine

Article Title: In vivo analysis of vascularization and biocompatibility of electrospun polycaprolactone fibre mats in the rat femur chamber

doi: 10.1002/term.2868

Figure Lengend Snippet: HE staining, van Gieson staining, and immunohistochemical detection of CD 34: Representative histological stainings (a–o) 20 days after implantation into the femur chamber in Lewis rats. (a–c) HE staining of (1) granulation tissue, (2) implant, and (3) bone. (d–f) HE staining with higher magnification. (g–i) Collagen fibres were detected by van Gieson staining. (j–l) The presence of endothelial cells and accordingly the presence of vascular structures were confirmed by immunohistochemical detection of CD34. (m–o) Negative controls. Areas of the implant are marked (#), and arrows denote vascular structures [Colour figure can be viewed at wileyonlinelibrary.com ]

Article Snippet: For detection of capillaries, endothelial cells were immunohistochemically stained using a rabbit anti‐rat CD34 antibody (Hoelzel Diagnostika Handels GmbH, Koeln, Germany).

Techniques: Staining, Immunohistochemical staining